Sunday, September 13, 2015

Red bean extract reduces inflammation and increases survival in a murine sepsis model

Siwoo Park, Ki-Choon Choi, Minghao Fang, Yung Chul Lim, Young-Mi Jeon, Jeong-Chae Lee 

Abstract:
This study used in vitro and in vivo experimental models to investigate the anti-inflammatory potential of the butanol fraction of red bean ethanol extract (BF-RBEE), which contains the biologically active molecule catechin-7-ß-d-glucopyranoside. Treatment with BF-RBEE inhibited nitric oxide production in lipopolysaccharide (LPS)-stimulated macrophages through suppression of extracellular signalregulated kinase and I?Ba activation. In an in vivo murine sepsis model, oral administration of BF-RBEE improved mouse survival. Specifically, the survival rate of mice injected with LPS was 0 and 40% in ICR and BALB/c mice, respectively, whereas the survival of mice co-treated with BF-RBEE was 100% in both mouse types. This increase in survival with the BF-RBEE administration was correlated with decreased tumor-necrosis factor-a and interferon-? and increased interleukin-10. Oral administration of BF-RBEE also inhibited total and ovalbumin-specific IgE production in experimental mice. These results suggest the possible usefulness of red beans in the treatment of inflammatory diseases.

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Keywords:
red bean, sepsis, lipopolysaccharide, ovalbumin, IgE

 
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