Wol Soon Jo, Yoo Jin Choi, Hyoun Ji Kim, Byung Hyouk Nam, Sook Hee Hong, Gye An Lee, Sang Wha Lee, Su Yeong Seo, Min Ho Jeong
Abstract
The aim of the present study was to examine the anti-inflammatory activities of extracts fromTetraselmis suecica with respect to nitric oxide (NO) production, tumor necrosis factor (TNF)-α and interlukin (IL)-6 release in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. We prepared methanolic extracts and water extract using protease. Of all the prepared extracts, 80% methanol extract exhibited the strongest anti-inflammatory effect. Thus, we further characterized the hexane fraction (He-TS) and ethyl acetate fraction (EA-TS) that contained potential bioactive compounds from the 80M-TS fraction of T. suecica. Both He-TS and EA-TS fractions resulted in a significant and dose-dependent inhibition of LPS-induced NO production, TNF-α, and IL-6 release (p<0.05). This strong inhibition of various inflammatory mediators such as NO, TNF-α, and IL-6 by EA-TS fraction from T. suecica suggests that this fraction might contain a potential bioactive compound that would be a good candidate for the development therapeutic anti inflammatory agent.
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