Xihong Zhao, Li Wang, Jin Chu, Yanmei Li, Yanyan Li, Zhenbo Xu, Lin Li, Mark E. Shirtliff, Xiaowei He, Yao Liu, Jihua Wang, Liansheng Yang
Abstract
A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borneSalmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be 65°C for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of LAMP assays was performed on 214 food-borne Salmonella strains using a rapid procedure and easy result confirmation, where the specificity of LAMP and polymerase chain reaction (PCR) assays was 97.7% (209/214) and 91.6% (196/214), respectively; with a 100% specificity for both assays.
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