Cytoprotective activity of lotus (Nelumbo nucifera Gaertner) leaf extracts on the mouse embryonic fibroblast cell

Ju Yeon Ha, Kyueun Lee, Jeongmi Park, Ahyoung Dong, Han-Seung Shin

                                                                          

Abstract

This study was conducted to evaluate the cytoprotective activity of lotus (Nelumbo nuciferaGaertner) leaf extract (LLE) on mouse embryonic fibroblast (MEF) cells. The 2-diphenyl-1-picrylhydrazyl hydrate (DPPH) free radical scavenging activities of LLE increased in a concentration dependent manner. The cells, damaged by oxidative stress, decreased their viability following increasing concentration of H₂O₂, but the co-treatment of n-butanol fraction of LLE and H₂O₂ resulted in an increase in cell growth, by about 25%, compared to the cells treated with H₂O₂. The n-butanol fraction of LLE inhibited the cytotoxicity induced by H₂O₂ in a concentration dependent manner. The oxidative damage to the cells, measured by apoptotic and necrotic cell accumulation, was similar with the addition of the n-butanol fraction of LLE to H₂O₂. Taken together, these results suggest that LLE inhibited the cytotoxicity which is induced by H₂O₂, and has a protective effect on MEF cell against oxidative stress.

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Keywords :lotus leaf ,proximate composition ,antioxidant activity ,apoptosis ,cell viability.