Effects of ethanol extracts from stalked sea squirt (Styela clava) on antioxidant potential, oxidative DNA damage and DNA repair

Jae-Hee Park, Bo-Young Seo, Seung-Cheol Lee, Eunju Park

                                                                           

Abstract

The aim of this study was to assess the total radical trapping antioxidant potential and antigenotoxic effects by comet assay of ethanol extracts of stalked sea squirt, Styela clava, (tunic, substrate, and whole). All extracts of stalked sea squirt effectively scavenged ABTS^{· +} in a dose dependent manner. Pretreatment with each extract of stalked sea squirt produced significant reductions in oxidative DNA damage at concentrations of 1–50 μg/mL, with whole extract of stalked sea squirt showing higher inhibition (16.1 μg/mL) of H₂O₂ induced DNA damage than substrate or tunic extracts based on ED₅₀ values. The addition of 50 μg/mL of stalked sea squirt extracts to human leukocytes after oxidative stimulus (200 μM H₂O₂) for 5 min positively influences the kinetics of DNA repair during 24 hr of incubation. These results indicate that the ethanol extracts of tunic, substrate, and whole stalked sea squirt have significant antioxidant activities that protect against oxidative DNA damage and improve DNA repair capacity.

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Keywords :stalked sea squirt ,Styela clava ,total reactive antioxidant potential ,DNA damage ,DNA repair.