Mohammad Reza Nasri Nasrabadi, Seyed Hadi Razavi
Abstract
In an attempt to accumulate lycopene in the bacterium Dietzia natronolimnaea HS-1 cultured in beet molasses, the influence of lycopene cyclase inhibitors on carotenogenesis (within the range 0–60 ppm) were evaluated. All inhibitors blocked the biosynthesis of canthaxanthin and resulted in accumulation of lycopene as the predominant carotenoid. As the final step of a study aiming at the optimization of culture conditions, a central composite design to a fed-batch process was applied to optimize the concentrations of lycopene cyclase inhibitors (within the range 0–50 ppm) in order to achieve high level accumulation of lycopene from D. natronolimnaea HS-1 cultured in beet molasses. On the basis of this approach, the optimum concentrations of lycopene cyclase inhibitors required to achieve the highest level of lycopene accumulation (8.26±0.17 mg/L) were determined as follows: imidazole, 24.74 ppm; nicotinic acid, 28 ppm; piperidine, 24.05 ppm; pyridine, 27.6 ppm; and triethylamine, 23.22 ppm.
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