Sunday, April 13, 2014

Genetic determination and localization of multiple bacteriocins produced byEnterococcus faecium CWBI-B1430 andEnterococcus mundtii CWBI-B1431

Ana Aguilar-Galvez, Robin Dubois-Dauphin, David Campos, Philippe Thonart

                                                                                    

Abstract

Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431 from artisanal-produced Peruvian cheeses showed the presence of 4 putative bacteriocin genes: enterocin A, enterocin B, enterocin P, and mundticin KS. The multiple bacteriocin producer E. faecium CWBI-B1430 presented 1 plasmid of 34.6 kb, whereas E. mundtii CWBI-B1431 contained 1 plasmid of 11.0 kb. The structural gene responsible for mundticin KS production was located on 5.6 and 3.1 kbHindIII plasmid fragments. The reverse transcription-PCR analysis showed the expression of the bacteriocin genes enterocin A, enterocin B, and mundticin KS in E. faecium CWBI-B1430 and the bacteriocin genes enterocin P and mundticin KS in E. mundtii CWBI-B1431. To our knowledge, this is the first report of the expression of mundticin KS in E. faecium and enterocin P in E. mundtii.

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Keywords : Enterococcus faecium ,Enterococcus mundtii ,enterocin P ,mundticin KS ,genetic expression.
 
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