Gee-Woo Seo, Jeong-Yong Cho, Jae-Hak Moon, Keun-Hyung Park
Abstract
Four compounds obtained from the ethyl acetate-soluble neutral fraction of methanol extracts of the Welsh onion (Allium fistulosum L.) were purified by column chromatography using silica gel, octadecyl silane (ODS), and Sephadex LH-20 with a guided DPPH radical scavenging assay. After purification, the compounds were isolated by ODS-HPLC. The isolated compounds were identified as N-trans-feruloyl-3′-methoxytyramine (1), N-cis-feruloyl-3′-methoxytyramine (2), N-trans-p-coumaroyltyramine (3), and 3,5,7-trihydroxyflavone (kaempferol, 4) based on the spectroscopic data of NMR and MS. To the best of our knowledge, this is the first study to identify compound 1–3 in the Welsh onion. Compound 1 and 2 showed significantly (p<0.05) higher DPPH radical scavenging activities than compound 3.
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Keywords: Allium fistulosum,Welsh onion,cinnamic acid amide,DPPH radical,antioxidant.
