Jong Kook Kim, Jung In Kim, Nam Keun Lee, Young Tae Hahm, Moo Yeol Baik, Byung Yong Kim
Abstract
This work determined the characteristics of β-carotene produced from Rhodosporidium sp. isolated from citrus fruits, and an effective extraction method was established. To extract β-carotene from the isolated Rhodosporidium sp., the cell walls were destroyed using dimethyl sulfoxide (DMSO) solution with and without the use of glass beads and a sonicator. Extracted β-carotene was identified by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) with a β-carotene standard. The yields of β-carotene extracted in DMSO, DMSO and glass beads, and DMSO with a sonicator were 3.371, 5.112, and 3.301 μg/mL, respectively. Isolated β-carotene was relatively heat stable, with 80% of the viable molecules remaining at 80°C.
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Keywords:Rhodosporidium sp.,β-carotene,carotenoid
extraction,high performance liquid chromatography (HPLC).
